FIG. 2.

No evidence of oxidative stress in S_O2A flies. (A) The levels of hydrogen peroxide (H₂O₂) in whole tissue homogenate were compared between control flies and S_O2A flies. The H₂O₂ level was similar between the two groups in normoxia (p>0.05), but 3 days of hyperoxia treatment significantly increased H₂O₂ level in control flies (p<0.001) but not in S_O2A flies. Three days of hyperoxia treatment also significantly increased the accumulation of (B) malonaldehyde (MDA) (p<0.05) and (C, D) 2,4-dinitrophenylhydrazine (DNP) (p<0.01) in control flies, but not in S_O2A flies. *p<0.05, **p<0.01, and ***p<0.001; ^#p<0.05 when compared with S_O2A flies in hyperoxia.