FIG. 4.
Modification of mitochondrial functions in SO2A flies. Mitochondria from fly thoraces were isolated and the superoxide yield was determined using electron paramagnetic resonance (EPR) techniques. A representative EPR curve was shown in (A) and (B). (C) Superoxide production was significantly decreased in mitochondria from SO2A flies as compared to those from control flies in state 3 respiration (p<0.05) and a tendency of such a decrease was observed in state 4 respiration (p=0.07). (D) Oxygen consumption in isolated mitochondria was compared and SO2A flies exhibited a significantly decreased state 3 respiration (p<0.01) as compared to control flies. (E) Enzymatic assay in isolated mitochondria revealed that SO2A flies exhibit a significantly decreased complex I (p<0.05) and complex III (p<0.01) activity than control flies. *p<0.05 and **p<0.01. (To see this illustration in color the reader is referred to the web version of this article at www.liebertonline.com/ars).