. Author manuscript; available in PMC: 2013 Feb 28.

Published in final edited form as: Biochemistry. 2012 Feb 16;51(8):1658–1668. doi: 10.1021/bi201899b

Table 2.

Changes in substrate selectivity of E. coli KDPG aldolase mutants.^a

Mutants	Substrate selectivity compared to KDPG cleavage
	KHO	S-KHPB
Wild-type	1	1
T161S/S184L	250	2200
T161S/S184F	---	3400

Selectivity ratio = [(k_cat/K_M^KDPG)/(k_cat/K_M^X)]^Wild-type/[(k_cat/K_M^KDPG)/(k_cat/K_M^X)]^Mutant