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. Author manuscript; available in PMC: 2012 Mar 29.
Published in final edited form as: J Cell Biochem. 2009 Dec 15;108(6):1337–1345. doi: 10.1002/jcb.22364

Fig. 1.

Fig. 1

TAT-CC2 peptide binds to endogenous NEMO in OCPs and inhibits NEMO oligomerization. Wild-type (WT) and mutated (mut) CC2 peptides (50 μM) were added to cells and (A) stimulated with vehicle, RANKL, or TNF for 30 min. Lysates (lanes 1–6) were pre-cleared with IgG, immunoprecipitated with anti HA antibody and then blotted with anti NEMO antibody. Lane 7 represents a positive control (PC) for NEMO expressed in TNF-stimulated cells (crude lysate). Lower panel represents β-actin expression in equal concentrations of total protein from crude lysates collected from the respective samples before immunoprecipitation, used as loading control. B: Cells were treated with either WT-CC2 or mut-CC2 for the time points indicated. Cell lysates were processed for immunoblots in non-reducing conditions. Superscript numbers 1, 2, and 3 indicate NEMO monomer, dimer, or trimer, respectively.