Figure 6.

LRRK2 induced mitochondria fragmentation could be completely restored by dominant-negative DLP1. (A) Representative immunoblot confirmed the overexpression of DLP1 K38A in double-transgenic stable clonal cell lines. Equal protein amounts (10 μg) were loaded and tubulin was used as an internal loading control. (B) Representative pictures show that overexpression of DLP1 K38A mutant restores LRRK2-induced mitochondria fragmentation in double-transgenic cell lines. Green, tubulin; red, mito-DsRed2; blue, DAPi. Insets represent boxed areas. Quantification of mitochondria morphology showed a significant increase in the aspect ratio (C) and a decrease in the percentage of cells displaying fragmented mitochondria (D) in LRRK2 WT, R1441C and G2019S cells also expressing DLP1 K38A. For each cell line, at least 500 cells were analyzed in each experiment and experiments were repeated three times (*P< 0.05, when compared with the control cells; Student's t-test).