Comparison of the Efficacy of STTM with One Mutated Binding Site.
(A) Diagram of STTM with mutated miR165 binding site or mutated miR166 binding site. Orange indicates the spacer region. Blue indicates the bulge sequences in the miRNA binding sites. Red indicates mutations in the miRNA binding sites.
(B) Phenotypes of transgenic plants containing STTM with mutated miR165 binding site or mutated miR166 binding site. Bars = 1.0 cm.
(C) RNA gel blotting to determine the levels of miR165/166 in transgenic plants containing STTM constructs with mutated miR165 binding site or mutated miR166 binding site. Total RNAs were prepared from multiple primary transformants.