Figure 5.

GUS Activity in Pro_JUB1:GUS Lines.

(A) to (I) Arabidopsis.

(A) Ten-day-old seedling. GUS staining is mainly localized to cotyledons, the tip regions and margins of leaves representing the oldest but not yet senescent leaf regions, and primary and secondary roots.

(B) Ten-day-old seedling. Strong GUS staining located in tips of newly emerging leaves (arrows).

(C) and (D) Flowers at different development stages. GUS staining is virtually absent in unopened young flowers. In open flowers (D), GUS staining appears in mature anthers, filaments, and the stamen abscission zone.

(E) GUS staining is weak to absent in ~50% expanded leaves from soil-grown plants.

(F) Strong GUS staining is located in senescent regions of a partially senescent leaf from soil-grown plants.

(G) GUS staining in roots; note the absence of GUS activity in the meristematic zone.

(H) GUS staining is absent from emerging lateral roots (arrows).

(I) GUS staining in root cap (arrow). Staining was for ~1 h.

(J) to (P) Tobacco.

(J) Leaf, with more intense staining in the tip and margins. GUS activity is also visible around wound sites.

(K) and (L) Young flower without GUS staining.

(M) and (N) Open flower showing GUS staining in petal tips and anthers.

(O) Roots. Note the absence of GUS activity in the meristematic zone, whereas the root cap shows GUS staining (arrows).

(P) Absence of GUS activity from emerging lateral roots (arrows). Staining was for ~6 h.