Figure 7.

EST-Treated JUB1-IOE Plants Exhibit Increased Tolerance to NaCl Stress, Whereas Tolerance to NaCl Is Reduced in the jub1-1 Mutant.

(A) Two-week-old JUB1-IOE seedlings were transferred from solid MS medium to liquid MS medium containing 150 mM NaCl, in the absence (mock; 0.15% ethanol) or presence of 15 μM EST added to induce JUB1 expression. Plants were incubated for 72 h. EST-treated JUB1-IOE seedlings are more tolerant to salt stress.

(B) Higher chlorophyll levels are retained in EST-treated JUB1-IOE seedlings after salt treatment.

(C) Seedlings of wild-type (WT) plants are less affected by 72 h salt treatment (150 mM NaCl) than those of the jub1-1 mutant.

(D) Chlorophyll content remains higher in the wild type (WT) than jub1-1 plants after stress treatment. Data in (B) and (D) are the means of three biological replicates ± sd.