Table 2.
JUB1-Selected Binding Sequences
| JUB1-Selected Oligonucleotide (5′/3′) | JUB1 RBA | |
| Bio-RS-Oligo 1 | ||
| Pf15d21 | TCCCAATAGGATTCGTAAAGTGCCGTGTTCcgtccgccagcgcacc | 0.82 |
| Pf15d30 | AGCGAAGGATCAATTGAAGACGCCGTGATCcgtccgccagcgcacc | 0.74 |
| Pf15d25 | AATTTGACGTCATATTCTAACGCCGTAGTCcgtccgccagcgcacc | 0.83 |
| Pf15d64 | CAACATGAAGCTAGATGCCGTAGACcgtccgccagcgcacc | 1.18 |
| Bio-RS-Oligo 2 | ||
| Pf16d41 | TCACCCCCCCTTCTGAGGAACTCGGTGCCGTTCCTTTCcgtccacctgcag | 0.85 |
| Pf16d43 | CCTGTGACTTTCTCGAATCATGAGTGCCGTGCTCTCTCcgtccacctgcag | 0.48 |
| Pf16d44 | AGCGTATTCCCACTCCCGCTATGAGTGCCGTGCCCCCCcgtccacctgcag | 0.87 |
| Pf16d48 | TGCGAACCTTGTAGTGCTCCAGGATGCCGTACACCCCcgtccacctgcag | 0.84 |
| Pf16d82 | ATACTTTCCCCGAGTGTGATCGGGTGCCGTGCTCCCCcgtccacctgcag | 0.96 |
| Pf16d84 | CCATTTCGCCTTGCTGATTGCGCGGTGCCGTGTATCTCcgtccacctgcag | 0.96 |
| Pf16d88 | AAGCATTATCGTTGTTAAATACGGTGCCGTGTTCTGGCcgtccacctgcag | 0.66 |
| Pf16d98 | GGGCGGGCTGGTCTCGTATTGAGATGCCGTACTTGCCcgtccacctgcag | 0.92 |
| Pf16d100 | CCGATATCCTGTGAACTCAGCAAGATGCCGTCGTCCCCcgtccacctgcag | 0.89 |
| Pf16d102 | TTGGTGGTGCACGTATTTGATAGGGTGCCGTGTGTTCCcgtccacctgcag | 0.90 |
| Pf16d104a | ATGTTCGGCTGGATCTATATCACGATGCCGTGCGTTGCcgtccacctgcag | 1.20 |
| Pf16d106 | CCAATTCCCTTTTGCTGTTTAGTAGTGCCGTGCTGTCCcgtccacctgcag | 1.01 |
| Bio-RS-Oligo 3 | ||
| Pf17d51 | GGGACTTGTATACCTGTAAGGTGCCGTACCtcatgcggtacccacgtc | 0.87 |
| Pf17d52b | GGGAGGCCTCGTGCCAACCAGTGCCGTACGtcatgcggtacccacgtc | 1.07 |
| Pf17d54 | ACGTGATACACGCTCTATCAGTGCCGTGCCtcatgcggtacccacgtc | 0.85 |
| Pf17d57 | AGGCCGTTAAACATACATGAGTGCCGTACGtcatgcggtacccacgtc | 1.06 |
| Pf17d58 | ACACAATTGTGACGCGAAAGGTGCCGTACAtcatgcggtacccacgtc | 1.02 |
| Pf17d112 | CATCGGTTTCGGCCTTGTAGGTGCCGTACCtcatgcggtacccacgtc | 0.94 |
| Pf17d114 | TCCGTCCTCCGAGGATCATGATGCCGTACGtcatgcggtacccacgtc | 0.77 |
| Pf17d115 | TCAGGTACAACTCTGATGCAGTGCCGTACCtcatgcggtacccacgtc | 0.91 |
| Pf17d116 | CGAGCGTGGCCCAAAACACGGTGCCGTACCtcatgcggtacccacgtc | 0.94 |
| Pf17d117 | TCAGCTTGGCTGGAGCTAGGATGCCGTGGGtcatgcggtacccacgtc | 0.72 |
| Pf17d118 | GATCCCCCTCCTTGCCTCTAGTGCCGTACCtcatgcggtacccacgtc | 0.86 |
| Pf17d120 | TTCCCAGAACCTCTAACTGGATGCCGTACCtcatgcggtacccacgtc | 0.86 |
| Pf17d121 | GTACTAGATGCCGTACGtcatgcggtacccacgtc | 0.84 |
| Pf17d124 | AATGTCACTGTCCCCCTACAGTGCCGTGGCtcatgcggtacccacgtc | 0.91 |
| Pf17d126 | GGCTTAACCCGACAGCACAGACGCCGTGCCtcatgcggtacccacgtc | 0.94 |
| Pf17d127 | TGCCCAATGCCGTGTGTAGCACGCTGCCCA | 1.00 |
| Pf17d128 | AAATCCTTGTAAATCCCTAGATGCCGTACTtcatgcggtacccacgtc | 0.86 |
| Pf17d129 | GGTCGCACATCTCATCATGGATGCCGTACCtcatgcggtacccacgtc | 0.81 |
| Pf17d130 | ACGCACGTGTCTAGTATTGAGTGCCGTGCAtcatgcggtacccacgtc | 0.99 |
Thirty-six JUB1-selected oligonucleotides were obtained after five rounds of in vitro DNA binding site selection. Relative binding activity (RBA) of JUB1 to oligonucleotide Pf17d127 is set to 1. Values are based on a single assay. Nucleotides in lowercase letters are from flanking primer sequences. The JUB1 core binding sequence is in bold.