Figure 3.

JAZ8 Is Resistant to JA-Mediated Degradation in Vivo.

(A) Differential stability of JAZ8 and JAZ10.1 in the presence of JA. Transgenic seedlings (7 d old) expressing JAZ8-YFP or JAZ10.1-YFP fusion proteins were treated either with water (mock) or with 50 μM MeJA for the indicated amount of time prior to imaging of roots by confocal fluorescence microscopy. Image settings used for seedlings of the same genotype at different time points were identical.

(B) Coronatine stimulates JAZ8 turnover in a 26S proteasome-dependent manner. Transgenic seedlings (4 d old) expressing JAZ8-YFP were pretreated with water or the 26S proteasome inhibitor MG132 (100 μM) for 80 min, at which time seedlings were treated with coronatine (1 μM) for an additional 10 or 30 min. Seedlings treated with both coronatine and MG132 were imaged at the 30 min time point. YFP signal in root tissue was visualized by confocal microscopy. Microscope settings were identical for all images.

[See online article for color version of this figure.]