Figure 6.

The EAR Motif of JAZ8 Is Required for Repression of JA Responses.

(A) Consensus sequence of the Jas motif in JAZ8-like (top) and JAZ10-like (bottom) proteins from various plant species (see Supplemental Table 2 online).

(B) Consensus sequence of the N-terminal 15 amino acids from JAZ8-like proteins from diverse plant species (see Supplemental Table 2 online).

(C) Comparison of JA-mediated root growth inhibition in 35S:JAZ8 and 35S:JAZ8ΔEAR seedlings. Wild-type (WT), 35S:JAZ8 (line #24), and 35S:JAZ8ΔEAR (five independent homozygous lines) were grown for 8 d on medium supplemented or not supplemented with 20 μM MeJA. Root length ratios were calculated as described in the legend of Figure 4D. Data show the mean ± se for each genotype (n > 14 seedlings per genotype). Asterisks denote a significant difference (P < 0.05, Student’s t test) in comparisons between transgenic and wild-type seedlings. The mean root length ratio calculated for seedlings (n = 92) from all five independent 35S:JAZ8ΔEAR lines was not significantly different (P value = 0.068, Student’s t test) from that of the wild type.

[See online article for color version of this figure.]