In Situ Localization of MIL1 mRNA.
(A) to (E) Wild-type anthers hybridized with MIL1 antisense probe.
(A)
MIL1 transcripts are first detected in the stamen primordia, as indicated by the arrowhead.
(B) Transverse sections show that MIL1 is transcribed early in archesporial cells, as indicated by the arrowhead.
(C)
MIL1 is transcribed later in parietal and sporogenous cells.
(D) Dramatically enhanced MIL1 expression in inner parietal cells and microsporocytes.
(E)
MIL1 expression is stronger in tapetum and weaker in meiocytes.
(F) Control hybridization with MIL1 sense probe.
E, epidermis; Ms, microsporocyte; T, tapetum. Bars = 10 μm.