Figure 6.

Toluidine Blue–Stained Transverse Sections of Anthers in Both msp1-5 and the mil1 msp1-5 Double Mutant.

(A) to (D) msp1-5.

(E) to (H) mil1 msp1-5.

(A) and (E) msp1-5 and mil1 msp1-5 anthers develop normally at early developmental stage, with primary parietal cells and primary sporogenous cells.

(B) and (F) msp1-5 and mil1 msp1-5 both have excess microsporocytes (or SCPs) and almost two parietal layers.

(C) and (D) msp1-5 anthers have degraded microsporocytes, but intact parietal cells remained.

(G) and (H) mil1 msp1-5 SCPs behave like mil1 SCPs, increasing in number and decreasing in size as anthers develop, finally developing into somatic cells.

Ms, microsporocyte. Bars = 10 μm.

[See online article for color version of this figure.]