Figure 9.

TGA1’s Expression Pattern and Its Relationship with MIL1.

(A) Interaction of MIL1 and TGA1 in yeast grown on medium with X-gal, Gal, raffinose, and without Ura, Leu, Trp, and His. AD, pB42AD; BD, pLexA; MIL1, pLexA-MIL1; TGA1, pB42AD-TGA1.

(B) and (C) Wild-type (WT) anthers hybridized with TGA1 antisense probe. TGA1 is transcribed weakly in primary parietal and sporogenous cells (B), and later more strongly in microsporocytes and inner parietal cells (C).

(D) TGA1 hardly expresses in mil1 anthers.

(E) Wild-type anthers hybridized with TGA1 sense probe.

Ms, microsporocyte. Bars = 10 μm.