Distribution of Selected PE Molecular Species.
(A) to (C) Distribution of selected PE molecular species in mol %. Ion counts within ± 0.02 m/z units of each potassiated [M + K]+ (labeled on figure), protonated [M + H]+, and sodiated [M + Na]+ monoisotopic masses are normalized to the total PE ion signals on each pixel. A red-green linear color scale is used with a maximum mol % value corresponding to 50% of total PE for all images. See Supplemental Table 3 online for all PE species identified and m/z analysis for each species. Bar = 1 mm.
(D) Comparison of PE molecular species from MALDI-MSI sections and polar lipid fractions (purified through solid-phase extraction) of TLEs analyzed by direct infusion (n = 3).