Figure 10.

VIPP1-RNAi/amiRNA Strains Harbor Aberrant Structures at the Origin of Thylakoid Membranes.

(A) Electron microscopy image of a cell from VIPP1-amiRNA strain #18. Cells were grown at ~30 μE m⁻² s⁻¹ in TAP-NH₄ medium. An overview image is shown on the left, and zoom-ins of the regions demarcated by black boxes are shown on the right. Triangles indicate regions at the origin of multiple thylakoid membrane ramifications. CV, contractile vacuole; N, nucleus; P, pyrenoid; S, starch. Bars in overview images = 1 μm, those in zoom-ins = 0.2 μm.

(B) Electron microscopy image of a cell from the control strain. Cells were grown at ~30 μE m⁻² s⁻¹ in TAP-NH₄ medium. Symbols are as in (A).

(C) Electron microscopy image of a cell from VIPP1-RNAi strain #27. Cells were grown at ~30 μE m⁻² s⁻¹ in TAP-NO₃ medium. Abbreviations are as in (A).

(D) Electron microscopy image of a cell from VIPP1-RNAi strain #27. Cells were grown at ~30 μE m⁻² s⁻¹ in TAP-NH₄ medium. Abbreviations are as in (A).