Figure 2.
Examination of VIPP1/2 Levels in VIPP1-RNAi and -amiRNA Strains by Immunoblotting.
Transformants generated with empty vectors (control strains) or VIPP1-RNAi and VIPP1-amiRNA constructs in the cw15-325 (#12) and cw15-302 (#18) strain backgrounds, respectively, were grown in TAP-NH4 medium. Total protein from the transformants and purified recombinant VIPP1 and VIPP2 at the indicated protein concentrations were separated on 7.5 to 15% SDS-polyacrylamide gels and analyzed by immunoblotting using antisera against VIPP1 and VIPP2. HSP90C served as loading control.