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Suppression of Marker Genes Related to IT Formation and Nodule Organogenesis.
Total RNA was isolated from the control hairy roots (Ct) expressing the empty vector and representative plants expressing SIP2-RNAi-1 (L10, L11, L17, and L19) and SIP2-RNAi-2 (L23, L26, and L27). Real-time RT-PCR analysis was performed to assess the expression levels of SIP2, NIN, ENOD40-1, and ENOD40-2 genes. Error bars represent sd of the experimental values obtained from three technical replicates.
