Figure 3.
Comparable expression of wild-type hAPP does not increase hippocampal ENK and Penk expression. A, B, Hippocampal levels of hAPP mRNA (A right), Penk mRNA (A, left), Aβ1–x (B, left), and Aβ1–42 (B, right) in hAPPWT mice from line I5 and hAPPFAD mice from line J20 (n = 7–10 mice per genotype; age, 2–3 months). C, Coronal sections illustrating typical ENK immunostaining in 4- to 6-month-old mice of the indicated genotypes. D, Densitometric quantitation of ENK immunoreactivity revealed no significant differences between hAPPWT mice and age-matched NTG controls in all hippocampal subfields except the oml of the dentate gyrus. E, Levels of ENK immunoreactivity in the hilus did not correlate with hippocampal plaque loads in 6-month-old hAPPFAD mice. *p < 0.05, **p < 0.01, ***p < 0.001 (Student's t test). Values are mean ± SEM.