Figure 4.

NER dual incision activity on dG-AAF, dG-AF and dA-ALII containing plasmids. Plasmids containing site-specific dG-AAF (lane 1), dG-AF (lane 2) or dA-ALII (lanes 3–6) residues were incubated with a HeLa cell extract. Excision products containing were detected by annealing to complementary oligonucleotides with a 4G overhang, which served as a template for end-labeling with [α-³²P] dCTP and sequenase. The reaction products were resolved on a 14% denaturing polyacrylamide gel. A low molecular weight ladder from NER was used as a size marker and the position of 25 and 34 nt bands are indicated. Asterisks denotes non-specific, NER-independent bands.