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Transient absorption (675 nm) kinetics. The prompt absorbance increase in pure Zn-cyt c crystals (blue) is caused by the generation of *Zn-cyt c. In 2Fe:1Zn-cyt c cocrystals (red), there is an additional rise attributable to the Zn-porphyrin cation radical, Zn-cyt c+. The negative signal following the laser flash results from Zn-cyt c fluorescence. (Inset) A view of early kinetics from 2Fe:1Zn-cyt c cocrystals showing the rounded feature.
