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Real-time quantitative PCR amplification plots of each DNA template [(A) Ds-temp 1, (B) Ds-temp 4, (C) Cont-temp] and their linear standard curve analysis (D). PCR was performed with Deep Vent DNA pol (exo+) in the presence of 50 µM dDsTP and NH2-hx-dPxTP, 300 µM natural dNTPs, and different concentrations of DNA templates (20 fM, 200 fM, 2 pM, 20 pM).
