Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer extension assays with duplex DNA with varying length of 3′ single-stranded overhangs. The DNA sequences are shown beneath each set of lanes. For each overhang length, the first set of lanes is the duplex DNA substrate and the second set is the single-stranded extension of the primer used without the 14-mer template. In each case, all four dNTPs were supplied. The approximate numbers of nucleotide incorporations for several of the more prominent bands are shown.
