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. 2011 Dec 1;40(6):2470–2480. doi: 10.1093/nar/gkr1111

Figure 2.

Figure 2.

Generation of pop in-pop out Cbp1-minus mutant and a complementing Cbp1 mutant. (A) Analysis of pop-in and pop-out transformants of pK454 by PCR. (B) Analysis of the transformants of pC454 by PCR. C is the complemented band. (C). Western blot of the Cbp1 protein in S. islandicus. L—protein size ladders, W—wild-type, T—pop-in transformant, M—deletion mutant, CM—complemented mutant.