Figure 5.

Probing for pre-crRNAs and crRNAs and on a S. solfataricus P2 microarray. (A) Overexpression of Cbp1 resulted in growth retardation. Cell cultures in exponential growth phase were changed from glucose medium (GCVy) to arabinose medium (ACVy) at 0 h (T1) for induction of Cbp1 expression. CK—transformants carrying the empty vector and OE—overexpression of Cbp1. (B). Western blot of the Cbp1 at time intervals of 0, 4 and 20 h. (C) Differential hybridization yields of pre-crRNAs and mature crRNAs in the Cbp1 overexpression mutant relative to wild-type cells. The microarray carried oligonucleotide probes specific for selected spacer sequences of the CRISPR loci A–F. Northern blot analysis results showing band intensities of mature crRNAs that are consistent with the microarray results.