Table 2.
Absolute yield (%)a | ||||
---|---|---|---|---|
Substrateb | Inter.c | Intra.d | Total | % Inter.c |
ds U6U (16) | 31.9 ± 3.0 | 2.0 ± 0.7 | 33.9 ± 3.0 | 94.1 ± 1.9 |
ss U6U (18) | 3.4 ± 0.6 | 3.2 ± 0.2 | 6.9 ± 1.3 | 52.1 ± 13.5 |
ds A6A (17) | 26.1 ± 3.8 | 2.4 ± 1.6 | 28.6 ± 3.5 | 91.3 ± 6.0 |
ss A6A (19) | 5.4 ± 1.4 | 2.4 ± 0.6 | 8.0 ± 1.5 | 68.0 ± 4.5 |
Yields are the average of at least 3 separate experiments each carried out in triplicate.
The substrate is designated by its secondary structure (ss, ds).
Inter. corresponds to strand scission at the 5'-adjacent nucleotide from 6.
Intra. corresponds to strand scission at the nucleotide where 1 is generated.