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Deuterium isotope effect on the reactivity of 5,6-dihydrouridin-5-yl (1) in duplex RNA.
| KIEa | ||
|---|---|---|
| pH | C2'-2H | C3'-2H |
| 7.2 | 3.6 ± 0.7 (5) | 1.1 ± 0.1 (3) |
| 3.8 | 4.7 ± 0.8 (3) | 0.9 ± 0.1 (2) |
KIE determined by measuring cleavage in 5'-32P-16 relative to that in 5'-32P-20 or 5'-32P-21. Each experiment was carried out in triplicate and the number of experiments is in parentheses.
