Fig. 4.
Galanin and GALR2 are associated with arrest of cell proliferation. A, BrdU incorporation after galanin treatment. The BrdU-positive cells (left) were detected as described in “Materials and Methods” (magnification × 200). The BrdU incorporation rate is the ratio of BrdU-positive cell number to the total cell number. Closed bars indicate galanin treatment and open bars indicate no treatment (right) (**P<0.01). B, Galanin and GALR2 regulate cell cycle control genes. Serum-starved cells were treated with 100 nM galanin for 24 h, and the expressions of p27Kip1 (upper left), p57Kip2 (upper right), and cyclin D1 (lower) were assessed by immunoblotting. The band intensities were determined by densitometry and normalized to GAPDH (**P<0.01).