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. Author manuscript; available in PMC: 2012 Mar 29.
Published in final edited form as: Clin Cancer Res. 2009 Mar 10;15(7):2222–2230. doi: 10.1158/1078-0432.CCR-08-2443

Fig. 5.

Fig. 5

Induction of apoptosis in galanin-treated UM-SCC-1-GALR2 cells. A, Annexin-V staining. The annexin-V-positive cells were stained and counted by flow cytometry (left). Percent positive is the ratio of annexin-V-positive cells to total cell number (right) (**P<0.01). B, DNA fragmentation analysis. Genomic DNA was isolated and electrophoresed on a 2 % agarose gel. The DNA ladders were visualized under UV light with ethidium bromide. Lane M: Size marker. Lane 1: DNA isolated from UM-SCC-1-mock cells without galanin treatment. Lane 2: UM-SCC-1-mock cells with galanin treatment. Lane 3: UM-SCC-1-GALR2 cells without galanin treatment. Lane 4: UM-SCC-1-GALR2 cells with galanin treatment.