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AFM images of DbpA–RNA complexes. (Bar = 100 nm.) Globular structures at the end of the RNA molecule represent protein signals. The mean end-to-end contour length is 135 ± 4 nm, which is consistent with the free dsRNA. Visualization of protein signals at the end of the RNA molecule indicates that DbpA requires an ssRNA or moderate fork junction for binding before performing the unwinding activity.
