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. 2012 Mar 29;8(3):e1002566. doi: 10.1371/journal.pgen.1002566

Figure 6. Essential function of Treacle in mitotic spindle formation and mitotic progression.

Figure 6

(A) PCR analysis of efficacy of TCOF1 knockdown in HeLa cells 48 hours after control (siGL2) or TCOF1 (si TCOF1) siRNA transfection. (B) Immunostaining of mitotic HeLa cells in control knock-down (siGL2) and TCOF1 knock-down (si TCOF1) cultures analyzed with anti-α-tubulin (red) and anti-Treacle (green) antibodies. (C) Mitotic cells in TCOF1knock-down (siTCOF1) and control (siGL2) cultures immunostained with anti-α-tubulin (red) and anti-centrin (green) antibodies. Arrowheads indicate abnormal mitotic spindle and chromosome alignment in TCOF1 knock-down cultures. (D) Graph depicting the frequency of mitotic cells labeled via immunostaining with a pH 3 antibody at 6–16 hours post siGL2 or siTCOF1 transfection and the marked delay in mitotic exit exhibited by TCOF1 knock-down cells. Scale Bars: B and C, 5 µm.