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. 2012 Mar 29;8(3):e1002602. doi: 10.1371/journal.pgen.1002602

Figure 7. Small molecule and genetic inhibition of crIs4 [unc-54p::clp-1] induced paralysis.

Figure 7

(A) Treatment with 5 µM nemadipine-A reduced crIs4 [unc-54p::clp-1] induced paralysis in egl-19(ad695); crIs4 and hlh-1(cc561ts); crIs4 animals. % paralyzed animals for each strain are indicated with (+) or without (−) nemadipine-A treatment. Significance of difference based on Student's T-test compared to egl-19(ad695); crIs4 (*, P<0.001) or hlh-1(cc561ts); crIs4 (**, P = 0.001) without nemadipine-A treatment. (B) Treatment of hlh-1(cc561ts); crIs4 animals with empty RNAi feeding vector (L4440), clp-1, or asp-1 to asp-6 (RNAi). Significance of difference compared to hlh-1(cc561ts); crIs4 fed on empty vector (RNAi) is based on Student's T-test (*, P<0.001; **, P<0.05). Numbers shown represent the mean ± SEM from at least 5 independent experiments, which involved counting day 2 adults from synchronized worm populations (see Methods). n>60 per experiment.