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. 2012 Mar 29;8(3):e1002621. doi: 10.1371/journal.ppat.1002621

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Blasco et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunoblot analysis of 10 µg of culture filtrate (CF) and 5 µg of cell lysates (CL) of wild-type H37Rv (left) and H37RvΔRD1 (right) strains grown for 4 days after transfer into Sauton's medium without Tween-80. GroEL2 was used as a control for autolysis, SodA as a loading control for CF and CL samples and EsxA as a control for ESX-1-dependent secretion. (B) Immunoblot analysis of H37Rv CL shown in (A) fractionated into cell wall/cell membrane (W/M) and cytosolic (CYT) components by ultracentrifugation. GroEL2 was used as a loading control. (C) Immunoblot analysis of Erdman CF (20 µg) for each of the time points indicated.