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. 2012 Mar 29;8(3):e1002591. doi: 10.1371/journal.ppat.1002591

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Dennis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–F) Electron microscopy of gonads from 15°C adults; regions of the gonad are as indicated at the top of the panels. Capsids localize predominantly to the core in the mid-pachytene region, but concentrate near nuclei in late-pachytene germ cells (C–E) and in oogonia (F). Note that many capsids localize with microtubules (arrowheads) both in the core (panel A) and in germ cells (panel C). P granules are visible in panels C (arrow) and in panel D; arrowheads in panel D indicate examples of nuclear pores. (E) Cluster of capsids near the nucleus of an early oogonium. (F) A “crescent” of capsids by an oogonium nucleus; there are at least 64 capsids and numerous microtubules visible at higher magnifications of this single thin section (data not shown). Scale bars: A–F (0.2 µm).