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. 2011 Dec 17;12:480. doi: 10.1186/1471-2105-12-480

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Copyright ©2011 Risso et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Yeast dataset: Read count vs. GC-content. Lowess fits of gene-level log(count + 1) vs. GC-content for the eight YPD lanes from the Yeast dataset, after FQ between-lane normalization. Curves are colored according to culture/library preparation. The GC-content effect is the same for lanes assaying the same culture/library preparation, but can be different for lanes assaying different cultures/library preparations. Figure S4 displays the scatterplot and lowess fit for the first YPD lane (culture/library preparation Y1, flow-cell 428R1).