Figure 2. Mechanism of inhibition of Chembridge compound 7759844 against both substrates IMP and NAD⁺.

The purified Mt-GuaB2 enzyme was incubated at 37°C for five minutes with substrate in the absence and presence of different concentrations of compound 7759844 and the reaction started by addition of NAD⁺ or IMP. The initial velocity data were plotted against three different IMP (left panel) or NAD⁺ concentrations (right panel) and varying inhibitor concentrations. The data represent the mean +/− standard error of duplicate experiments. The 1/V versus 1/[Sub] plot at the inset (top) of each graph only depicted the type of inhibition.