Figure 7. Vpu-induced rpr upregulation, DIAP1 downregulation and apoptosis in the wing disc depend on JNK pathway activity.

(A–E) rpr-lacZ expression (X-gal staining) in en-Gal4/+ (A) bsk¹/en-Gal4 (B), bsk¹/en-Gal4; UAS-Vpu/+ (C), en-Gal4/+; UAS-bsk-IR/+ (D) and en-Gal4/+; UAS-Vpu UAS-bsk-IR/+ (E). (F) XY confocal section of Vpu (green), DIAP1 (red) immunostaining and F-actin network (gray) of a dpp-Gal4/UAS-bsk IR UAS Vpu wing imaginal disc. (G–H) XY confocal section of Vpu immunodetection (H′,H; green), TUNEL labeling (G′,H″,H′″; red) and F-actin network (H″″; gray) of dpp-Gal4/UAS-bsk-IR (G) and dpp-Gal4/UAS-Vpu UAS-bsk-IR (H) wing imaginal discs. Below each image in F–H: transverse XZ section (f′-f′″, g′ and h′-h″″) through the plane indicated by the line in F′, G′ and H′″, respectively. The epithelial sheet is not disorganized (arrowheads in f′″ and h″″) by Vpu-expression when bsk is concomitantly knocked-down by RNAi.