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. 2012 Mar 29;7(3):e28056. doi: 10.1371/journal.pone.0028056

Figure 3. Accurate routing of DNA using mesofluidic devices.

Figure 3

(1) Using the mesofluidic pump, four radiolabeled DNA sequences (ZA–A1, ZB–B2, ZC–C10, and ZD–D7) were hybridized to an anticodon array filled with 96 oligonucleotide-conjugated resins. The four spots at well positions A1, E19, G13, and O3 of the array (indicated by red boxes) were filled with resins A1′, B2′, C10′ and D7′ respectively. (2) Hybridized DNA was transferred to an anion-exchange chemistry array using a mesofluidic Southern blotter. (3) The DNA was eluted from the anion-exchange chemistry array, pooled, and split again using an anti-codon array containing the same 96 resins in a different order: well positions G9, G15, I9, and I15 of the array (indicated by red boxes) were filled respectively with the A1′, B2′, C10′, and D7′ resins. The arrays were imaged at each step.