Figure 7. Expression of the amp genes in PAOΔampR.

RNA was isolated from cells exposed to β-lactam antibiotic, reverse transcribed to cDNA and tested in triplicate by qPCR with gene-specific primers, as described in the text. The expression of ampC (β-lactamase), ampD, ampDh2, and ampDh3 (amidases), ampG and ampP (permeases), and nagZ (glycoside hydrolase) were tested in the ampR mutant relative to PAO1. Values have been normalized to expression in PAO1 under the same conditions (log₁₀ RQ = 1) and bars above or below the line represent up- and down-regulation, respectively. * p value<0.05.