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. 2012 Mar 29;7(3):e34067. doi: 10.1371/journal.pone.0034067

Figure 10. Gene expression in PAOΔampR at 40 minutes and 2 hrs post-β-lactam exposure.

Figure 10

RNA was isolated from PAO1 and PAOΔampR cells exposed to β-lactams for either 40 minutes or 2 hours and reverse transcribed to cDNA. The expression of the sigma factor rpoS, lecA and lecB (galactophilic lectin genes known to be RpoS-regulated), and mexT (MexEF-OprN efflux pump regulator that is not RpoS-regulated) were tested by qPCR with gene-specific primers, as described in the text. Values have been normalized to expression in PAO1 under the respective conditions. ** p-value<0.006; *** p-value 0.0002 as determined by unpaired t test.