Table 3. Effect of deletion of ampR on QS-regulated virulence phenotypes.
Strain | Pyocyanin productiona | LasA activityb | LasB activityc | |||
Uninduced | Inducedd | Uninduced | Inducedd | Uninduced | Inducedd | |
PAO1 | 22.31±0.18 | 24.30±1.10 | 0.37±0.005 | 0.39±0.004 | 1.65±0.25 | 1.54±0.15 |
PAOΔ ampR | 2.29±0.11e | 5.74±0.56f | 0.26±0.002 | 0.19±0.002g | 0.5±0.03h | 0.57±0.08i |
Pyocyanin concentrations were expressed as micrograms of pyocyanin produced per microgram of protein.
LasA activity was expressed as change in OD600 per hour per microgram of protein.
LasB elastase activity was expressed as change in OD495 per microgram of protein.
Induction was carried out with 100 µg/ml of penicillinG.
p-value<0.0001 when comparing PAO1 and PAOΔampR.
p-value<0.0001 when comparing PAO1 and PAOΔampR.
p-value of 0.02 when comparing PAO1 and PAOΔampR.
p-value of 0.01 when comparing PAO1 and PAOΔampR.