|
Strains
|
|
|
|
Escherichia coli
|
|
|
| DH5α |
General purpose cloning strain; Δ(lacZ)M15
|
New England Biolabs |
| OP50 |
Food source for culturing C. elegans
|
Caenorhabditis Genetics Center |
| DBS7 |
DH5α harboring pEXG2 carrying the 1520 bp ΔampR fragment (pDBS7) |
This study |
| DBS220 |
DH5α harboring the 944 bp promoterless ampR ORF cloned into pCR2.1 TOPO (pDBS220) |
This study |
| DBS271 |
DH5α containing the 944 bp ampR ORF subcloned as a EcoRI-SacI fragment from pDBS220 into pMMB67EHGm |
This study |
|
Pseudomonas aeruginosa
|
|
|
| PAO1 |
Wild-type |
[42]
|
| PKM314 |
PAO1 with pMMB67EH-Gm (empty vector) |
This study |
| PKM315 |
PAOΔampR; In-frame deletion of ampR (PA4109) |
This study |
| PKM316 |
PAOΔampR with pMMB67EH-Gm (empty vector) |
This study |
| PKM317 |
PAOΔampR (pAmpR); ampR ORF on pMMB67EH-Gm (pDBS271) moved into PAOΔampR; IPTG-inducible; GmR; ampR complementing clone |
This study |
| PAOretS::aacC1
|
Gm insertion mutant of retS; hyper biofilm-former |
S. Lory |
| PAOΔpelΔpslΔalgD
|
In-frame triple deletion of the algD ORF and pel and psl operons; biofilm non-former |
S. Lory |
|
Staphylococcus aureus
|
|
|
| DBS116 |
Wild type S. aureus used for LasA assays |
Lab collection |
|
Plasmids
|
|
|
| pCR2.1 TOPO |
TA cloning vector for PCR products; ApR, KmR; ColE1 f1 ori lacZα
|
Invitrogen |
| pRK600 |
Helper plasmid for conjugation |
[133]
|
| pEXG2 |
Allelic exchange vector; GmR, colE1 ori, oriT, sacB
|
[131]
|
| pMMB67EH-Gm |
GmR; IncQ, RSF1010, lacIq Ptac expression vector |
[134]
|
| pDBS7 |
pEXG2 with the ΔampR fragment, flanked by EcoRI and BamHI sites |
This study |
| pDBS220 |
pCR2.1 TOPO containing the 944 bp ampR ORF, PCR amplified from the PAO1 genome using DBS_ampRF2 and DBS_ampRR2 |
This study |
| pDBS271 |
pMMB67EH-Gm containing the ampR ORF, subcloned as an EcoRI-SacI fragment from pDBS220 |
This study |
