Table 2. Summary of test results of DNA microarray and DGGE assays on 51 clinical tissue samples and 9 cultures from field samples.
Sample ID | Sample type | DGGE | DNA microarray | Comment |
100SR10 | ovine, lung | M. arginini, M. ovipneumoniae | M. arginini 3, M. ovipneumoniae | concordant, dual infection |
34 B 10 | bovine, lung | M. bovis, M. alkalescens | M. bovis, M. alkalescens | concordant, dual infection |
108 B 10 | bovine, lung | M. bovis, M. alkalescens 1 | M. bovis, M. alkalescens 1 | concordant, dual infection |
13SR11 | ovine, nasal swab | M. ovipneumoniae, M. arginini | M. ovipneumoniae, M. arginini 3 | concordant, dual infection |
33F11 | culture, ovine, milk | M. agalactiae | M. agalactiae, M. putrefaciens | more species by AS |
19B10 | bovine, lung | M. alkalescens, M. bovis | M. alkalescens, M. bovis, M. dispar 2 | more species by AS |
490 B 09 | bovine, vaginal swab | M. bovigenitalium | M. bovigenitalium 3, M. alkalescens 1 | more species by AS |
485 B 09 | bovine, vaginal swab | M. bovigenitalium | M. bovigenitalium, M. alkalescens 1 | more species by AS |
53B10 | bovine, swab | M. bovirhinis | M. bovirhinis, M. bovis 1, M. arginini 2 , 3 | more species by AS |
365B10 | bovine, nasal swab | M. bovirhinis, M. dispar | M. bovirhinis, M. dispar, M. bovis 1, M.arginini 2 , 3 | more species by AS |
36B10 | bovine, lung | M. bovis | M. bovis, M. arginini 1 ,4 | more species by AS |
49B10 | bovine, lung | M. bovis | M. bovis, M. dispar 1 | more species by AS |
279B11 | bovine, lung | M. bovis | M. bovis, M. dispar 1 | more species by AS |
669 B 09 | bovine, eye swab | M. bovoculi | M bovoculi, M. canadense | more species by AS |
128SR09 | ovine, eye swab | M. conjunctivae | M. conjunctivae, M. ovipneumoniae 1, M. arginini 2 | more species by AS |
32 B 10 | bovine, lung | M. dispar | M. dispar, M. bovis 1, M. alkalescens 1, M. bovirhinis 2 | more species by AS |
39 B 10 | bovine, lung | M. dispar | M. dispar 1, M. bovis 1, M. alkalescens 1 | more species by AS |
265B10 | bovine, nasal swab | M. dispar | M. dispar, M. arginini 2 , 3 | more species by AS |
83A10 | culture from strain collection | M. meleagridis | M. meleagridis, M. dispar 2 | more species by AS |
120A10 | chicken, eyelid | M. synoviae | M. synoviae, M. iners | more species by AS |
95SR10 | ovine, swab | unidentified bands | M. conjunctivae | AS more specific |
79O10 | caprine, lung | M. arginini, M. ovipneumoniae 2 | M arginini 3 | more species by DGGE |
142 B 09 | bovine, lung | M. bovirhinis, M. alkalescens 1 | M. bovirhinis | more species by DGGE |
89 B 10 | bovine, lung | M. bovirhinis, M. alkalescens 1 | M. bovirhinis, M. dispar 1 | discordance in second agent |
36 samples | tissue (29) and culture (7) | M. agalactiae, M. alkalescens, M. arginini, M. bovirhinis, M. bovis, M. canadense, M. capricolum subsp. capricolum, M. conjunctivae, M. gallisepticum, M. mycoides subsp. capri, M. ovipneumoniae, M. putrefaciens, M. synoviae | concordant, monoinfections |
confirmed by species-specific PCR,
species-specific PCR negative,
not distinguishable from M. gateae.