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. 2012 Mar 29;7(3):e33313. doi: 10.1371/journal.pone.0033313

Figure 5. Expression of ICAM-3, RUNX1 and RUNX3 during macrophage differentiation, monocyte transendothelial migration and DC maturation.

Figure 5

A. Left, ICAM-3 and FOLR2 mRNA expression levels along M-CSF monocyte-derived-macrophages, as determined by qRT-PCR at the indicated time points. Results are expressed as Relative mRNA levels (relative to GAPDH mRNA levels and the ICAM-3 and FOLR2 mRNA level on monocytes). Data represent mean ± SD of 3 independent donors (*P<0.05 compared with ICAM-3 mRNA level of monocytes). Right, ICAM-3, RUNX1 and RUNX3 expression on monocytes and M-CSF-polarized macrophages, as determined by Western blot at the indicated time points. As a control, GAPDH expression levels were also determined. The experiment was performed twice and one of the experiments is shown. B. Left, ICAM-3 mRNA expression levels of monocytes (Mo.) and transendothelial migrated monocytes (Migrated Mo.), as determined by qRT-PCR. Results are expressed as Relative mRNA levels (relative to GAPDH mRNA levels and the ICAM-3 mRNA level on monocytes). Data represent mean ± SD of 3 independent donors. Right, ICAM-3, RUNX1 and RUNX3 expression on monocytes (Mo. and transendothelial migrated monocytes (Migrated Mo.), as determined by Western blot at the indicated time points. As a control, GAPDH expression levels were also determined. The experiment was performed twice and one of the experiments is shown. C. Left, ICAM-3 mRNA expression levels of DC either untreated (−) or treated with 10 ng/ml of LPS during 24 h (+) as determined by qRT-PCR. Results are expressed as Relative mRNA levels (relative to GAPDH mRNA levels and the ICAM-3 mRNA level on untreated DC). Data represent mean ± SD of 3 independent donors. Right, ICAM-3 and RUNX3 expression on DC either untreated (−) or treated with 10 ng/ml of LPS during 24 h (+), as determined by Western blot. As a control, GAPDH expression levels were also determined. The experiment was performed twice and one of the experiments is shown.