Figure 4. B cell development molecules are expressed at intermediate levels by CD27^lo cells.

(a) Intermediate levels of many B cell development molecules in CD27^lo cells suggest a transient phenotype consistent with developing pre-plasmablasts engaged in receptor editing before differentiation (n = 6 subjects, SAM analysis q values in Supplementary Table 1). (†) at least one probe significantly different between CD27^lo and CD27^hi at FDR = 0.03, SAM analysis, (*)CD27^lo/Undivided, at least one probe significant, FDR = 0.01, (**)CD27^lo/undivided at least one probe significant at FDR = 0.008. (b) Quantitative RT-PCR confirmed relative RNA levels of BACH2, CD27, PRDM1 (BLIMP-1), XBP-1, IgL, and HSPA5 (BiP) (n = 10 subjects, paired T-test p values in Supplementary Table 2). The point in each violinplot is the median value and the brackets indicate the interquartile range.