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. Author manuscript; available in PMC: 2012 Mar 30.
Published in final edited form as: Brain Res. 2007 Jun 18;1192:99–113. doi: 10.1016/j.brainres.2007.06.007

Figure 1. Endogenous expression pattern of Vsx1 and Chx10 in postnatal retina.

Figure 1

(A-C) The specificity of Vsx1 antibody was determined by immunohistochemistry (A, B) and by western blot analysis (C) on retinal samples from wild type (+/+) and Vsx1τlacz/τlacz (τlacz/τlacz) mice at P14 or older. The nuclear pattern of Vsx1 staining in the INL is observed in wild type (A) and absent in Vsx1τlacz/τlacz retina (B). (C) A band of the expected size (approximately 39 kDa) is present in wild type and absent in Vsx1τlacz/τlacz P14 retinal lysates. Another band of approximately 64 kDa is observed in both lysates. β-Actin was used as a loading control. (D-I) Confocal images showing Vsx1 (D, G) and Chx10 (E, H) expression patterns in P8 wild type retina. Merged images are shown in F and I. Arrows point to examples of cells with high Vsx1 and low Chx10 expression. Arrowheads point to examples of cells with low Vsx1 and high Chx10 expression. The open arrowhead points to a cell that co-expresses both proteins at low levels. Asterisks in H and I indicate a staining artifact. Scale bars: 40 μm. Abbreviations: ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.