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Map of pBudVP2-VP1 and pBudVP2-VP1/VP22. pBudVP2-VP1 was constructed by cloning of the CAV VP1 gene into the NotI and KpnI of EF-1α MCS and VP2 gene into the SalI and BamHI sites of CMV MCS of pBudCE4.1. To generate pBudVP2-VP1/VP22, the MDV-1 VP22 DNA fragment was inserted into the KpnI and XhoI cloning sites of the constructed pBudVP2-VP1 plasmid in the frame with the CAV VP1 gene.
