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. 2011 Mar 14;8:119. doi: 10.1186/1743-422X-8-119

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Copyright ©2011 Moeini et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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In vitro expression analysis of the constructs in chicken MSB1 cells by RT-PCR (A and B) and Western blotting (C). (A) RT-PCR results for VP1 and VP2 (lanes 1 and 2, respectively) in the pBudVP2-VP1-transfected cells. (B) RT-PCR results for VP1, VP2 and VP22 (lanes 1-3) in the pBudVP2-VP1/VP22-transftected cells; M: GeneRuler™DNA Ladder Mix (Fermentas, Canada). (C) Western blotting results by using chicken anti-CAV (lanes 1-3) or anti-His (lanes 4 and 5) in the cells transfected with pBudVP2-VP1/VP22 (lane 1 and 5), pBudVP2-VP1 (lane 2 and 4) or in non-transfected cells (lane 3).