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Indirect immunofluorescence analysis. MSB1 cells were transfected with the recombinant plasmids by using Lipofectamine 2000 reagent and 2 days after transfection, transient expression of the encoded proteins was explored by indirect immunofluorescence using primary anti-CAV polyclonal antibody. (A) Cells transfected with pBudVP2-VP1; (B) Cells transfected with pBudVP2-VP1/VP22; and (C) Cells transfected with the parental plasmid.
