Table 1.
Primers for (A): PCR amplification and RT-PCR analysis of the viral genes; (B): RT-PCR analysis of chicken cytokines
| Gene | Accession Number | Sequence (5' to 3') | Expected product size (base pair) | |
|---|---|---|---|---|
| A: | ||||
| CAV VP1 | AF285882 | Forward Reverse |
CTAACGCGGCCGCACCATGGCAAGACGAGCTCGC CTAGGGGTACCCCAGTACATGGTGCTGTTGG |
1335 |
| CAV VP2 | AF285882 | Forward Reverse |
GCTAAGTCGACACCATGCACGGGAACGGC CATGGGGATCCCACTATACGTACCGGGGC |
648 |
| MDV-1 VP22 | AY311498 | Forward Reverse |
CATGGGGTACCATGGGGGATTCTGAAAGGC GTACGCTCGAGTCGCTATCACTGCTACGAT |
728 |
| B: | ||||
| IL-4 | NM_001007079 | Forward Reverse |
AGCTCTCAGTGCCGCTGATG TAGCTAGTTGGTGGAAGAAGG | 321 |
| IL-6 | NM_204628 | Forward Reverse |
ATGAACTTCACCGAGGGCTGC ACGGTCTTCTCCATAAACGAAG | 680 |
| IL-12 | AY262752 | Forward Reverse |
ACACATCTGATGAAGCACTGCC TTGGGATATGTCCAGGTC-ACAG |
598 |
| β-actin (positive control) |
EU931581 | Forward Reverse |
ATGTGCAAGGCCGGTTTCGC TCCTCAGGGGCTACTCTCAG |
254 |